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Salvia Divinorum Extraction |
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Written by Valdez L.J, et al
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Tuesday, 12 August 1997 |
Salvia divinorum
Experimental Section Collection, Extraction and Isolation. Live specimens of S. divinorum were collected at Cerro Quemado (Sep 3, 1979) and Cerro Rabon (March 7, 1980) in Oaxaca, Mexico. The plants were cultivated at the Mattaei Botanical Gardens, The University of Michigan, in order to provide material for research. Fresh Salvi leaves (5.350 kg) were lyophilized and forced through 7- and 16-mesh screens yielding 674.1 g of powdered dry material. The powder was extracted in 30-40 g lots for 24 hr with ethyl ether (1 L/lot) using Soxhlet apparatus and dried in vacuo, giving a total of 27.51 g of ether extract. The extract was partitioned between hexanes (600 mL) and 90% aqueous methanol (600 mL) for 48 h using a liquid/liquid extractor and yielded, after removal of the solvent in vacuo, a 7.41 g methanol fraction. The hexane fraction was repartitioned as above and the combined concentrated methanol fractions (9.36 g) were subjected to further purification by flash column chromatography. [The 9.36 g of methanolic fraction eventually yielded 893 mg of pure Salvinorin A] Ref.
Valdez L.J. et al; Divinorum A, a Psychotropic Terpenoid, and Divinorin B from the Hallucinogenic Mexican Mint Salvia divinorum; J. Org. Chem. 1984, 49, 4716-4720.
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